茶树越冬芽在休眠与萌发时期的物质交流变化及其分子调控

为揭示不同萌发物候型茶树的休眠机制,以特早生茶树品种龙井43和中生茶树品种碧云为材料,利用钙黄素处理茶树茎段,检测越冬芽在休眠与萌发时期与其他器官的物质交流情况。利用同源比对鉴定胼胝质水解相关基因,并分析其序列特征及在冬季不同时期的表达模式。结果表明,越冬芽在茶树生长阶段和休眠阶段都存在着与着生茎段和母叶间的物质交流;从茶树越冬芽休眠形成到解除的不同时期,其物质交流存在"强-弱-强"的变化规律,但龙井43的与碧云相比存在较短的物质交流减弱时期;两种茶树的物质交流变化模式与鉴定到的茶树胼胝质水解正向调控相关基因CsGLU1的表达模式密切相关;启动子序列分析进一步证实CsGLU1启动子区有多个与激素信号以及低温和休眠响应相关转录因子结合的保守序列。茶树越冬芽在休眠和非休眠状态下都存在与茎和母叶之间的物质交流,且物质交流强弱与茶树越冬芽休眠状态改变密切相关。CsGLU1可能是参与胼胝质水解调控,改变茶树越冬芽物质交流水平,进而影响茶树休眠状态的关键基因。这对明确茶树越冬芽休眠状态变化和深入揭示不同萌发物候型茶树休眠机理有重要意义。     

Field Evaluation of Cocksfoot,Tall Fescue and Phalaris for Dry Marginal Environments of South‐Eastern Australia. 1. Establishment and Herbage Production

Newly developed candidate cultivars of cocksfoot (Dactylis glomerata L.), phalaris (Phalaris aquatica L.) and tall fescue (Festuca arundinacea Schreb. = syn. L. arundinaceum (Schreb) Darbysh.) were evaluated over four years for persistence and productivity against current commercial cultivars in small plots at five locations selected for lower and less reliable rainfall and difficult soils (low pH and high Al) in south‐eastern Australia known to be marginally too dry for these grass species. The five locations were ‐ representing summer dominant rainfall, Inverell, in northern New South Wales (NSW); ‐ representing uniform rainfall; Trungley Hall, (medium rainfall), and Beckom (lower rainfall) both in southern NSW; and; representing a winter dominant pattern ‐ Eversley, (higher rainfall), and Bealiba, (lower rainfall), in central Victoria. The objective was to determine if the new candidate cultivars were more likely to persist and to be productive than current commercial cultivars. The study showed that most phalaris and cocksfoot treatments were highly productive in high rainfall years at one or both sites in southern NSW. However, all treatments had become much less productive by the end of the experimental period due to plant loss under hot, dry conditions in the final summer. At Bealiba in central Victoria, cocksfoot was the most productive species with several cocksfoot treatments of both subsp. hispanica and subsp. glomerata still present at the final harvest despite a hot and dry final summer‐autumn. Tall fescue was the most productive species in the two higher rainfall environments (Inverell, Eversley) although most treatments of all species performed well at those sites. Continental tall fescues were more productive on average than Mediterranean tall fescues at the strongly acidic Eversley site. As a result of this work, two of the new cocksfoot candidates (Moroccan Fine and AVH48 Selection) and one of the tall fescue candidates (Summer Active 1) have been licensed for commercial development and release.     

气候变暖对新疆不同秋眠级紫花苜蓿种植适宜性的影响

基于新疆102个气象台站1961?2019年逐日平均气温、最低气温资料,采用统计学方法以及ArcGIS的空间插值技术,在对影响新疆紫花苜蓿种植的主要气候因子 ≥ 5 ℃积温、冬季最低气温时空变化特征分析的基础上,以80%保证率 ≥ 5 ℃积温和90%保证率冬季最低气温作为指标因子,研究了气候变暖背景下,新疆不同秋眠级紫花苜蓿种植气候适宜性的变化。结果表明：新疆80%保证率 ≥ 5 ℃积温和90%保证率冬季最低气温的空间分布总体呈现“南疆多(高),北疆少(低);平原多(高),山区少(低)”的格局。受上述两指标要素空间分异的综合影响,秋眠级4～6级紫花苜蓿适宜种植区主要分布在南疆的塔里木盆地以及东疆的吐鲁番盆地和哈密盆地;秋眠级2～3级紫花苜蓿适宜种植区分布在塔里木盆地、吐鲁番盆地和哈密盆地周边山前倾斜平原,伊犁河谷以及准噶尔盆地西部和东部的低平原地带;秋眠级1～2级紫花苜蓿适宜种植区分布在北疆北部、西部,准噶尔盆地腹地以及天山和昆仑山中、低山带;阿尔泰山、天山和昆仑山区大部为紫花苜蓿不适宜种植区。影响不同秋眠级紫花苜蓿种植的主导因子不同,秋眠级4～6级紫花苜蓿适宜种植区以及紫花苜蓿不宜种植区主要受80%保证率 ≥ 5 ℃积温的影响;秋眠级1～2级和2～3级紫花苜蓿适宜种植区主要受90%保证率冬季最低气温的影响。在全球变暖背景下,近59年新疆 ≥ 5 ℃积温和冬季最低气温分别以65.52 (℃·d)·10 a?1和0.63 ℃·10 a?1的倾向率呈极显著(P < 0.001)上升趋势,80%保证率 ≥ 5 ℃积温和90%保证率冬季最低气温也总体增多或升高,但其变化具有阶段性差异,时段Ⅰ (1961–1980年) 80%保证率 ≥ 5 ℃积温和90%保证率冬季最低气温为近59年最低时期;时段Ⅱ(1981?2000年)较时段Ⅰ的90%保证率冬季最低气温升高2.9 ℃,但80%保证率 ≥ 5 ℃积温变化不大;时段Ⅲ(2001?2019年)较时段Ⅱ的90%保证率冬季最低气温不升反降0.8 ℃,而80%保证率 ≥ 5 ℃积温增加147.0 ℃·d。受上述气候要素变化的影响,近59年新疆秋眠级4～6级和2～3级紫花苜蓿适宜种植区呈扩大趋势,而秋眠级1～2级紫花苜蓿适宜种植区及紫花苜蓿不宜种植区呈减小趋势。这表明气候变暖对新疆紫花苜蓿种植的发展总体趋于有利。     

牧草柳枝稷种子破休眠技术的研究

柳枝稷switchgrass (panicum virgatum)的种子属深度休眠。本文探索了后熟化(干藏),冷冻层化(湿润冷藏),磨擦,浸泡,以及植物生长调节剂GA和ABA对破除种子休眠的影响。     

Effects of smoke,ash and heat shock on seed germination of seven species from Central Zagros rangelands in the semi-arid region of Iran

Fire products have been widely identified as germination cues for a number of species from both fire-prone and fire-free ecosystems. However, there is uncertainty about the effects of fire products on seed germination of semi-arid rangeland species. We examined the germination of seven dominant species from Central Zagros rangelands in the semi-arid region of Iran under various heat shock (60, 80 and 120 °C), aerosol smoke, ash and control treatments. Our findings showed that fire products could enhance, inhibit or not affect the seed germination of rangeland species in this area. The germination percentage of Astragalus adscendens increased following 60 °C heat treatment in comparison with the control. The germination of Astragalus verus, Bromus tectorum, B. tomentellus and Phlomis olivieri were increased significantly using smoke treatment (P<0.0001). The seed germination of B. tomentellus and B. tectorum was promoted by ash treatment, whereas the other species exhibited no response or were inhibited by fire products. In addition, smoke treatment shortened the overall germination time of B. tectorum. These findings suggest that fire products affect the germination of several species and can serve as potential tools for understanding the vegetation dynamics and restoration of disturbed semi-arid rangelands.     

桃芽休眠诱导过程中IAA、ABA含量及PpPINs基因表达的变化

为认识桃芽休眠诱导过程中生理与相关基因表达的变化,分别用HPLC检测‘中农红久保’、‘中农3号’、03-30-035 3个桃品种(系)休眠诱导时期叶片和叶芽IAA和ABA含量,用RT-q PCR检测叶片和叶芽生长素极性运输载体基因Pp PIN1、Pp PIN3、Pp PIN5和Pp PIN8共4个基因的相对表达量。结果表明,在桃芽休眠诱导过程中,叶片IAA含量未见显著变化,ABA含量自10月6日开始升高,至10月16日含量达到高峰;叶芽中10月上中旬IAA含量骤增,而ABA含量逐渐降低。本研究中桃基因组的4个Pp PINs基因家族成员中,叶片中活跃表达为Pp PIN5,其次为Pp PIN3,在10月上中旬Pp PIN5出现强烈表达;而在10月26日,叶芽中活跃表达的Pp PIN1和Pp PIN3表达量骤降。桃休眠诱导过程中叶片和叶芽内内源激素和Pp PINs基因表达量变化明显不同。     

分叉管流动的数值模拟

采用非结构、非均匀的网格和有限元方法,通过求解Navier-Stokes方程,对90°分叉管的流动进行了并行数值模拟的研究。计算网格为500000个有限单元,计算Reynolds数为1500。计算结果给出了90°分叉管详细的流动结构。     

宁夏农民中专学历教育探析

结合工作实际,从宁夏农民接受教育的现状入手,分析了农民学历教育存在的一些问题,探索了解决问题的办法。     

Effect of Temperature on the Induction of Bud Dormancy in Ecotypes of Betula pubescens and Betula pendula

The purpose of this study was to determine the influence of temperature applied during short day-induced budset on induction of dormancy in six ecotypes of Betula pubescens Ehrh. and two ecotypes of Betula pendula Roth. Seedlings were grown in a phytotron at constant temperatures of 9–21°C under a 12 h photoperiod (SD) during dormancy induction. Induction of dormancy was monitored by following bud flushing and shoot growth after transfer to long photoperiod conditions (24 h) at 18°C. Chilling requirement was studied in seedlings exposed to 10 weeks of SD. In both species induction of bud dormancy developed most rapidly at 15–18°C, and both 9–12°C and 21°C delayed the induction of dormancy. Raising the temperature (from 9 to 21°C) applied during induction of dormancy significantly increased the chilling requirement. These responses were noted for all ecotypes tested, but in general the northern ecotypes entered dormancy more quickly than the southern ones. No such trend was recorded for chilling requirement, although a B. pubescens ecotype from Iceland and another from the coast of northern Norway appeared to require a longer chilling treatment than the other ecotypes. In conclusion, induction and depth of bud dormancy in birch are significantly affected by temperature conditions and these effects may explain some of the annual variation in dormancy and chilling requirement observed in nature.